When a PCR enrichment step is necessary, lower adaptor-ligated DNA inputs leads to greater amplification yields, hiding the latent disparity between kits. Detailed ddPCR data also reveal that the adaptor ligation yield itself varies by more than a factor of 10 between kits, certain ligation efficiencies being so low that it could impair the original library complexity and impoverish the sequencing results. The overall library preparations efficiencies of the libraries show important variations between the different kits with the ones combining several steps into a single one exhibiting some final yields 4 to 7 times higher than the other kits. We also investigated the potential influence of DNA input and DNA fragment size on the final library preparation efficiency. This method allows the precise quantification of fragments bearing either adaptors or P5/P7 sequences on both ends just after ligation or PCR enrichment. We compared 9 commercially available library preparation kits in a systematic manner using the same DNA sample by probing the amount of DNA remaining after each protocol steps using a new droplet digital PCR (ddPCR) assay. When it comes to preparing DNA sequencing libraries for Illumina, the current market leader, a plethora of kits are available and it can be difficult for the users to determine which kit is the most appropriate and efficient for their applications the main concerns being not only cost but also minimal bias, yield and time efficiency. The emergence of next-generation sequencing (NGS) technologies in the past decade has allowed the democratization of DNA sequencing both in terms of price per sequenced bases and ease to produce DNA libraries. Quantitation of next generation sequencing library preparation protocol efficiencies using droplet digital PCR assays - a systematic comparison of DNA library preparation kits for Illumina sequencing.Īigrain, Louise Gu, Yong Quail, Michael A Data are reported on the availability of schedule flexibility, job exchange, part-time arrangements, and leaves, as well as information about faculty status, tenure,… This report presents the results of a survey of Association of Research Libraries (ARL) members about the current climate and practices regarding flexible work arrangements. This survey was conducted to delineate the growing relationship between marketing and public relations and library funding by identifying: (1) the extent to which North American research libraries have developed organized programs in the areas of marketing and public relations (2) who holds positions in these areas and (3) the impact these…įlexible Work Arrangements in ARL Libraries. Marketing and Public Relations in ARL Libraries. This survey was designed to collect data and document some of the… The roles and responsibilities of the library systems officer continues to change as libraries move beyond the automation of library functions to offering resources in electronic formats and electronic access to information about collections beyond the walls of the home institution.
A review of the responses from 64 of the 94 ARL… Office of Management Studies.Īt the request of the Pennsylvania State University Library, the Office of Management Studies surveyed Association of Research Libraries (ARL) members requesting information and documentation illustrating the organization, nature, and level of the library instruction function at their institutions. The results of a survey of Association of Research Libraries (ARL) libraries (n=73) conducted in Fall 1989 are presented as well as examples of travel policies and guidelines submitted by the following respondents: the Universities of…Īssociation of Research Libraries, Washington, DC. This kit examines the methods and policies currently used to provide travel funding for academic librarians. The kit draws upon documents gathered as part of a 1986 Quick- SPEC survey that dealt with food and… This Systems and Procedures Exchange Center ( SPEC) flyer/ kit addresses some of the needs of Association of Research Libraries (ARL) members regarding library building use and the aesthetics and well-being of the materials and people in the building.
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