We can show the correlation between two parameters using a bivariate histogram, or cytogram, in the form of a dot, contour or density plot ( Figure 1.2). To display data from a single parameter, we can use a univariate histogram ( Figure 1.1).
Whatever the program used, the principles of data analysis are the same. There are a variety of programs supplied for this purpose some of them are sold commercially, others are free. It is convenient to have a program for analysis of data files on computers in other locations. The computer program can then be used to analyse data subsequent to its acquisition off-line analysis is useful for the preparation of illustrations for publications, lecture slides, etc. If the flow cytometer can sort cells, the computer controls the sorting process.Īs data are acquired, they written to the hard drive to create a file of data, often referred to as ‘listed data’. draw regions and set gates (see below) to be used during data acquisition.select histograms and cytograms for display.adjust the settings for colour compensation (see Chapter 5.2).select and adjust the threshold (discriminator) settings (see Chapter 2.5.1).select logarithmic or linear amplification.adjust the gain settings on the amplifiers.select area, width or height on different parameters (for pulse processing, see Chapter 2.5.2).The computer program controls the cytometer during data acquisition. All flow cytometers have a computer associated with them.
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